The California mouse (Peromyscus
californicus) is a monogamous species of rodent found along the
coastal mountain ranges and eastern Sierra Nevada of California. It
is the largest species in its genus with adult body length ranging
between 22-29 cm and body mass between 33-54 g. Adults are distinctly
bicolored with yellowish brown or gray mixed with black dorsal
coloring and a white underside and feet. Captive colonies of
California mice have been useful for the study of the neuroendocrine
mechanisms of parental behavior, aggression, and behavioral responses
to stress.
Species: Peromyscus californicus
Stain: Nissl
Fixation: [3540, 3547] Immersion in 5% acrolein
in phosphate buffered saline (PBS); [8372] Transcardial perfusion with PBS
followed by 4% PFA
Plane: coronal
Area: whole-brain
Source: [3540, 3547]
Rebecca Hao, Natalia Duque-Wilckens, and Brian Trainor;
[8372] Nou Vang, Adele Seelke, and Brian Trainor
Section thickness: 40 um
Notes: [3540, 3547] After fixation each brain was
immersed in 20% sucrose in PBS at 4 C overnight. Brains were then
rinsed in PBS before freezing on dry ice. Brains were sectioned at 40
um on a cryostat and then every third section was stained from just
posterior to the olfactory bulb through the cerebellum. [8372] After fixation
this brain was immersed in 40% PFA at 4 C overnight. The brain was then
transferred to 20% sucrose for 1-2 days before being rinsed in PBS and freezing
on dry ice. Brains were sections at 40 um on a cryostat and then every fourth
section was stained from just posterior to the olfactory bulb through the
cerebellum.
Mouse 3540 was a 3 month old male weighing 39.5 g;
Mouse 3547 was a 3 month old female weighing 36.5 g;
Mouse 8372 was a 38 day old male weighing 24.2 g.
