We also demonstrate that while the beta 2S- and beta 2L-subunit messenger RNAs frequently co-localize in many brain areas, certain structures (e.g., the ectostriatum, the hippocampus, the nucleus solitarius, the nucleus isthmi, pars parvocellularis, the Nucleus Isthmi, pars magnocellularis, the paleostriatum primitivum, the Purkinje cell layer, and the deep cerebellar nuclei) exclusively or predominantly contain either the beta 2S- or the beta 2L-subunit transcript. 
While the two transcripts are found to be colocalized throughout the chick neuroaxis, certain nuclei (for example, the Nucleus Isthmi, pars magnocellularis, the nucleus isthmi, pars parvocellularis, the nucleus solitarius and the paleostriatum primitivum) are found to contain predominantly either the gamma2S- or the gamma2L-subunit mRNA.
Both labelled nucleus spiriformis lateralis and nucleus geniculatus lateralis, pars ventralis especially heavily and also labelled the nucleus habenula medialis; nucleus subpretectalis; Nucleus Isthmi, pars magnocellularis; nucleus reticularis gigantocellularis; nucleus reticularis lateralis; nucleus tractus solitarii; nucleus vestibularis dorsolateralis; nucleus vestibularis lateralis; nucleus descendens nervi trigemini; and the deep cerebellar nuclei.
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