The main aim of the study was to investigate whether group II muscle afferents contribute to the inhibition of dorsal spinocerebellar tract (DSCT) neurons and thereby modulate information provided by these neurons in the cat.
Recent studies have indicated that the glycine receptor antagonist strychnine and the gamma-aminobutyric acid type A (GABA A) receptor antagonist bicuculline reduced the rapid-eye-movement (REM) sleep-specific inhibition of sensory inflow via the dorsal spinocerebellar tract (DSCT).
(1) Ipsilateral hemiataxia following dorsolateral medullary infarctions results from a lesion of the dorsal spinocerebellar tract and the inferior cerebellar peduncle conveying afferent information from the ipsilateral arm and leg.
The dorsal spinocerebellar tract (DSCT) provides a major mossy fiber input to the spinocerebellum, which plays a significant role in the control of posture and locomotion.
All of them presented a small infarction on the lateral surface of the caudal medulla corresponding to the dorsal spinocerebellar tract (DSCT).
Previous work from this laboratory has shown that activity in the dorsal spinocerebellar tract (DSCT) relates strongly to global hindlimb kinematics variables during passive displacements of the hindlimb.
Neurons of the dorsal horn (mainly Clarke's column) make up a dorsal spinocerebellar tract and neurons of the ventral horn (mainly spinal border cells) are at the origin of a ventral spinocerebellar tract.
Fibers labeled from unilateral injections into the paramedian lobule were found on the same side in the dorsal part of the lateral funiculus (DLF), corresponding to the dorsal spinocerebellar tract (DSCT), but contralaterally in the ventral part of the lateral funiculus (VLF) and in the ventral funiculus (VF), corresponding to the ventral spinocerebellar tract (VSCT).
Peripheral nerve-evoked potentials recorded in the cerebellum 35 yr ago inferred that sensory transmission via the dorsal spinocerebellar tract (DSCT) is reduced occasionally and only during eye movements of active sleep compared with wakefulness or quiet sleep.
dorsal spinocerebellar tract (DSCT) neurons receive converging sensory inputs from muscle, skin, and joint receptors and their cerebellar projection is a product of the spinal sensory processing of movement-related information.
The use of 100 microm serial sections through the SCA2 patient's central somatosensory components showed that obvious neuronal loss occurred in nearly all of the relay stations of this system (Clarke's column; cuneate, external cuneate and gracile nuclei; spinal, principal and mesencephalic trigeminal nuclei; ventral posterior lateral and ventral posterior medial nuclei of the thalamus), whereas the majority of interconnecting fibre tracts (dorsal spinocerebellar tract; cuneate and gracile fascicles; medial lemniscus; spinal trigeminal tract, trigeminal nerve and mesencephalic trigeminal tract) displayed signs of atrophy accompanied by demyelinization.
Until recently it was assumed that the information transmitted by the dorsal spinocerebellar tract (DSCT) was organized to represent single muscles or single joints in the ipsilateral hindlimb.
During the state of active sleep (AS), Clarke's column dorsal spinocerebellar tract (DSCT) neurons undergo a marked reduction in their spontaneous and excitatory amino acid (EAA)-evoked responses.
The superficial dorsolateral column (DLC(0-300)) was included in this analysis because it was hypothesized that large dorsal spinocerebellar tract fibres could also be activated by SCS.
dorsal spinocerebellar tract (DSCT) neurons transmit sensory signals to the cerebellum that encode global hindlimb parameters, such as the hindlimb end-point position and its direction of movement.
The present study was performed to provide evidence that dynamic neural processes underlie the reduction in dorsal spinocerebellar tract and spinoreticular tract neuron activity that occurs during active sleep. To ascertain the effect of local inhibition on the spontaneous and glutamate-evoked spike discharge of sensory tract neurons, preliminary control tests were performed during the state of quiet wakefulness, where GABA or glycine was co-administered in a sustained fashion during pulsatile release of glutamate to dorsal spinocerebellar tract (n=3) or spinoreticular tract (n=2) neurons. Extracellular recording experiments combined with juxtacellular application of glutamate were then performed on 20 antidromically identified dorsal spinocerebellar tract and spinoreticular tract neurons in the chronic intact cat as a function of sleep and wakefulness. The glutamate-evoked activity of a group of 10 sensory tract neurons (seven dorsal spinocerebellar tract, three spinoreticular tract), which exhibited a significant decrease in their spontaneous spike activity during active sleep, was examined. In contrast, the glutamate-evoked activity of a second group of eight sensory tract neurons (four dorsal spinocerebellar tract, four spinoreticular tract), which exhibited a significant increase in their spontaneous spike activity during active sleep, was not significantly altered in a state-dependent manner.These data indicate that, during natural active sleep, a dynamic neural process is engaged onto certain dorsal spinocerebellar tract and spinoreticular tract neurons, which in turn dampens sensory throughput to higher brain centers..
The dorsal spinocerebellar tract (DSCT) system is considered in detail as a model system that may be considered as an end point for the processing of proprioceptive sensory information in the spinal cord.
We tested this by recording from dorsal spinocerebellar tract (DSCT) and Purkinje cells under the same experimental conditions in which the hindlimbs of anesthetized cats were passively moved through a series of step-like movement cycles.
The dominating effect on neurones tentatively classified as dorsal horn dorsal spinocerebellar tract neurones was facilitatory from both skin areas.
The question we address here is the contribution of muscle tension afferent information in dorsal spinocerebellar tract (DSCT) sensory representations of foot position.
We studied the effect of these constraints on the activity in 70 dorsal spinocerebellar tract (DSCT) neurons using a multivariate regression model, with limb axis length and orientation as predictors of neuronal activity.
Spinocerebellar-tract cells in the dorsal horn receive synaptic connections from serotoninergic axons, but little is known about the relationships between serotoninergic axons and dorsal spinocerebellar tract (DSCT) cells in Clarke's column.
This paper reports the effect of limb movement speed on dorsal spinocerebellar tract (DSCT) activity recorded while the cat hindlimb was passively moved through two types of foot trajectories (figure eight and step cycle) at different speeds.
The transfer of the former is facilitated but that of the latter is either facilitated or depressed; it is facilitated via Clarke's column dorsal spinocerebellar tract neurons and depressed via dorsal horn dorsal spinocerebellar tract neurons.
Among other areas, neurons were labeled in the central cervical nucleus, the nucleus centrobasalis, Clarke's nucleus, the dorsal horn dorsal spinocerebellar tract area, the spinal border region, and Stilling's nucleus.
One week after a unilateral transection of the dorsal spinocerebellar tract at Th9-10, Py-IR in the Clarke's nucleus ipsilateral and caudal to the lesion was reduced by approximately 40%.
dorsal spinocerebellar tract (DSCT) neurons have been shown to transmit signals related to hindlimb position and movement direction in the anesthetized cat.
The dorsal spinocerebellar tract (DSCT) of the thoracic cord (the thoracic DSCT) consists of uncrossed ascending axons originating from Clarke's column, marginal neurons of Clarke's column, and lamina V neurons, and crossed ascending axons originating from neurons in lamina VIII and the ventromedial part of lamina VII.
dorsal spinocerebellar tract (DSCT) neurons in Clarke's column in the lumbar spinal cord of cats anesthetized with alpha-chloralose were recorded intracellularly.
Spinocervical tract, postsynaptic dorsal column and dorsal spinocerebellar tract neurons located in Clarke's column and in the dorsal horn were identified by their axonal projections.
Antigen was found in the spinal cord gray and white matter sensory neuronal circuits of nociception (the spinothalamic tract) and proprioception (the dorsal spinocerebellar tract and gracile fasciculus).
We report here about the modulation of dorsal spinocerebellar tract (DSCT) activity by limb posture.
In the present study, we asked whether axons of the dorsal spinocerebellar tract (DSCT) are capable of such growth.
We showed previously that neurons in the dorsal spinocerebellar tract (DSCT) may encode whole-limb parameters of movement and posture rather than localized proprioceptive information.
The present study used extracellular recording methods in chronically instrumented intact behaving cats to monitor the activity of lumbar dorsal spinocerebellar tract (DSCT) neurons within Clarke's column during the states of wakefulness, quiet sleep, and active sleep.
We compared the activity in the mossy fiber projection, the dorsal spinocerebellar tract (DSCT) with the responses of Purkinje cells in the spinocerebellum for evidence of correlated activity.
Clarke's column neurons of the dorsal spinocerebellar tract (DSCT) were recorded intracellularly in anaesthetized cats during weak sustained contractions of triceps surae (TS) produced by direct electrical stimulation of the muscle.
Effects of ionophoretic application of serotonin and of one of its agonists were tested on responses of dorsal horn dorsal spinocerebellar tract neurons evoked by electrical stimulation of peripheral nerves. It is proposed that facilitatory actions assist in maintaining tonic discharges of dorsal spinocerebellar tract neurons in some movements and that the selective control of group II input is used to correlate activity of spinal and supraspinal neurons. Both actions may be subserved by tight contacts between serotoninergic nerve fibres and dorsal spinocerebellar tract neurons, which have been revealed in a parallel study..
Contacts between serotoninergic nerve fibres and dorsal horn dorsal spinocerebellar tract neurons were analysed in order to investigate the morphological basis of actions of serotonin upon dorsal spinocerebellar tract neurons. In a series of experiments dorsal spinocerebellar tract neurons were labelled with intracellularly injected rhodamine-dextran in the cat. In the second series of experiments dorsal spinocerebellar tract neurons were labelled by retrograde transport of Fluorogold injected into the cerebellum in the rat. Serotoninergic nerve fibres were found in apposition to cell bodies of all feline dorsal spinocerebellar tract neurons (n = 7) and of 75% of rat dorsal spinocerebellar tract neurons (n = 90).
1) The dorsal spinocerebellar tract (DSCT) originating ipsilaterally from the thoracic and upper lumbar segments ascends in the dorsolateral fasciculus.
Uncrossed neurons were located in and around Clarke's column and thus are cells of origin of the dorsal spinocerebellar tract (DSCT).
Spinocerebellar neurons that project in the dorsal spinocerebellar tract (DSCT) receive mono- and polysynaptic inputs from specific sensory receptors in the hindlimb, and they project mossy fiber terminals to the cerebellar vermis.
Monosynaptic connections from sensory receptors to the dorsal spinocerebellar tract are believed to have a significant role in the transmission of sensory information to the cerebellum. We explored this possibility by examining the responses of dorsal spinocerebellar tract neurons to inputs from muscle receptors. We concluded that both types of population response resulted from the muscle stretch and therefore the responses of dorsal spinocerebellar tract cells to these stimuli do not simply reflect the activity in specific classes of sensory receptors..
Orthodromic activation of nucleus Z neurons was tested in response to electrical stimulation of the ipsilateral dorsolateral funiculus (which includes the dorsal spinocerebellar tract) and/or the dorsal columns.
We examined the functional organization of the dorsal spinocerebellar tract (DSCT) and found that it is similar to that of a parallel distributed network having widespread connectivity among parallel elements.
N1 and N3 waves are considered to originate from Group I fibers and to be conducted by second-order neurons ascending the ipsilateral-dorsolateral funiculus (dorsal spinocerebellar tract) after synaptic delays.
It is concluded that S1 spinocerebellar neurones convey a similar type of information to that of dorsal spinocerebellar tract and ventral spinocerebellar tract neurones but integrate it in a different way..
It has been generally accepted that the dorsal spinocerebellar tract ascends in the dorsal half of the lateral funiculus and enters the cerebellum via the inferior cerebellar peduncle, whereas the ventral spinocerebellar tract ascends in the ventral half of it and takes the superior cerebellar peduncle route.
Monosynaptic excitatory postsynaptic potentials (EPSPs) evoked by hindlimb muscle nerve stimulation were recorded intracellularly from dorsal spinocerebellar tract (DSCT) neurones in Clarke's column, in close proximity to the central canal.
In normal control subjects, monoclonal antibody 6A2 showed specificity for neurons of the dorsal nucleus of Clarke, the cells of origin of the dorsal spinocerebellar tract.
The present experiments were carried out to investigate the morphology and somatotopic location of dorsal spinocerebellar tract (DSCT) neurons that receive monosynaptic group 1 afferent input from hindlimb ankle extensor muscles in the cat.
Component 1 is likely to be due to the stimulation of group 1 muscle afferents which terminate in the dorsal horn and activate second-order neurones, many of whose axons go to form the ipsilateral dorsal spinocerebellar tract.
We have developed a model in order to analyze the factors eventually responsible for the strong negative serial dependency between successive interspike intervals in the discharge of the dorsal spinocerebellar tract (DSCT) neurons.
Since the ascending spinal axons which project to nucleus Z are collaterals of dorsal spinocerebellar tract fibres, it was possible to stimulate the parent axons at their termination in the anterior lobe of the cerebellum.
Excitatory postsynaptic potentials (EPSPs) evoked by impulses in single group I muscle afferents were recorded intracellularly in dorsal spinocerebellar tract (DSCT) neurones in the spinal cords of anaesthetized cats.
Component 1 is likely to be due to the stimulation of group 1 muscle afferents which terminate in the dorsal horn and activate second order neurones, many of whose axons go to form the ipsilateral dorsal spinocerebellar tract.
At 3 weeks, degeneration was also present in the cervical part of the dorsal spinocerebellar tract, in the lumbar part of the medial pontine-spinal tract, and in lamina VII in the lumbar ventral horn.
The projection fields of the dorsal spinocerebellar tract (DSCT) arising from Clarke's column, marginal neurons of Clarke's column, and lamina V neurons in the upper lumbar segments were studied by the anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) in the cat.
Impulse activity of 264 units of the dorsal spinocerebellar tract (DSCT) was recorded during random contraction or stretch in hindlimb muscles.
The discharges of 22 antidromically identified dorsal spinocerebellar tract (DSCT) neurones, whose activity was modulated in phase with the spontaneous respiratory cycle, were recorded in the lower thoracic segments of anaesthetized cats.
These results suggest that the ascending group I inhibitory pathway, formed by these interneurones, is associated specifically with the group I relay of the dorsal spinocerebellar tract in Clarke's column, rather than being conterminous with group I afferents, which project throughout the rostral lumbar and lowest thoracic segments..
Excitatory postsynaptic potentials (EPSPs) evoked by impulses in single group I muscle afferents were recorded in dorsal spinocerebellar tract (DSCT) neurons in the spinal cords of anesthetized cats.
In recent years the traditional pathway for position sense in the lower extremities has been challenged and it has been proposed that in the upper spinal cord the relevant fibres ascend not in fasciculus gracilis but in the dorsolateral part of the lateral funiculus with the dorsal spinocerebellar tract. To determine whether this premise is correct we have re-examined the clinical findings of five patients with spinal cord lesions and 62 patients who had undergone medullary tractotomy at a level where the dorsal spinocerebellar tract overlies the spinal tract of V. Of the five patients with spinal cord lesions, four had interrupted dorsal columns but intact dorsal spinocerebellar tracts. Another patient with a severed dorsal spinocerebellar tract and an incomplete lesion of the dorsal columns had no loss of position or vibratory sense but possibly some tactile impairment. These observations do not support the premise that position sense from the lower extremities is carried by fibres of the dorsal spinocerebellar tract at any level but do support the traditionally accepted pathway for position sense.
Axons considered to originate from these cells were located in the dorsal half of the lateral funiculus at the level of L2, intermingled with axons of the dorsal spinocerebellar tract originating at the levels of Clarke's column.
On the basis of their projection and termination, it is proposed that the axons of these dorsal horn spinocerebellar tract neurones contribute to the dorsal spinocerebellar tract (DSCT)..
We investigated the extent of the distribution of polysynaptic connections to the dorsal spinocerebellar tract (DSCT).
Fluorescent labels, injected into either the hindlimb muscles or the cerebellum, are retrogradely transported to motoneurones or dorsal spinocerebellar tract neurones respectively.
The synaptic connection between single group I afferents and dorsal spinocerebellar tract (DSCT) neurons in the cat spinal cord has been studied in an attempt to gain insight into the mechanisms of excitatory synaptic transmission in the mammalian CNS.
These reflex aspects are demonstrated in relation to the discharge of neurons in the dorsal spinocerebellar tract and of cerebellar cortical Purkinje cells in initial sinusoidal cycles. The intensity and phase advance of the discharge in dorsal spinocerebellar tract neurons is altered little, but these features are usually increased in Purkinje cells during initial stretches compared to continuous cycling.
Recent studies on the monosynaptic connection between primary afferent fibres and dorsal spinocerebellar tract (DSCT) neurones in the spinal cord of anaesthetized cats have been undertaken to investigate the mechanisms of excitatory synaptic transmission in the mammalian central nervous system.
As part of an investigation on excitatory synaptic transmission in the mammalian CNS, we have examined ultrastructural details of the synaptic connection between primary afferent fibers and dorsal spinocerebellar tract (DSCT) neurons in Clarke's column of the cat spinal cord.
The response characteristics of dorsal spinocerebellar tract (DSCT) neurons and ventral spinocerebellar tract (VSCT) neurons to the cutaneous inputs applied to footpads were studied in the cat.
The aim of this study was to identify nucleus z in the rat, to locate the cells of origin of spinal afferents to nucleus z, and to determine whether they are collaterals of the dorsal spinocerebellar tract. The location and extent of nucleus z were studied by filling the axon terminals of collaterals of the dorsal spinocerebellar tract (dsc) with horseradish peroxidase (HRP), which was injected into the inferior cerebellar peduncle. They were similar in location and morphology to neurons giving rise to the dorsal spinocerebellar tract, but were smaller in average diameter.
These sites--cochlear nuclei, ventral spinocerebellar tract and resciform body which includes dorsal spinocerebellar tract--are located outside the known locomotor regions.
It was possible using a collision test to show that afferent fibres synapsing on nucleus Z cells were collaterals of dorsal spinocerebellar tract cells.
The synaptic connection between primary muscle afferents and dorsal spinocerebellar tract (DSCT) neurons has been studied in an attempt to reveal some of the mechanisms underlying excitatory transmission in the mammalian central nervous system.
The role of the dorsal spinocerebellar tract (DSCT) in the neural control of normal interlimb coordination during overground stepping in adult cats was investigated using select spinal cord lesions.
In the same experiments, dorsal spinocerebellar tract (d.s.c.t.) neurones were retrogradely labelled by injection of horseradish peroxidase into the cerebellum.
Analysis of the afterpotential properties has been performed on various types of central neurones; spinal alpha-motoneurones, dorsal spinocerebellar tract cells, rubrospinal neurones and hippocampal CA1 pyramidal cells.
an ipsilateral dorsal spinocerebellar tract, presumably arising in dorsal root ganglion cells, and a larger ventral pathway, bilaterally arising in the spinal gray matter.
The aim of the study was to investigate whether inhibition of dorsal spinocerebellar tract (d.s.c.t.) cells evoked from group I afferents is mediated by the same interneurones which mediate the non-reciprocal inhibition of hind-limb motoneurones.
The topographical distribution of interneurones mediating disynaptic inhibition of dorsal spinocerebellar tract (d.s.c.t.) cells from group I muscle afferents in the cat was investigated using both physiological and morphological techniques.
The ascending volleys, which are supposed to represent mainly the action potentials of the dorsal spinocerebellar tract and to be elicited monosynaptically by collaterals of group I afferents, were essentially the same in the left tetanic and right control sides up to 5 days after toxin injection.
The results of these studies show that nerve fibers in the ipsilateral dorsal column, the ipsilateral dorsal spinocerebellar tract, and the contralateral ventrolateral tracts with respect to the side of leg stimulation, contribute to cortical SEP's. A lesion of the dorsal spinocerebellar tract affected only the early waves (less than 30 msec) of the SEP from leg stimulation ipsilateral to the side of the lesion, whereas a solitary lesion of the ventrolateral tract caused changes primarily in the amplitude of later waves (greater than 30 msec) of the SEP produced by contralateral leg stimulation.
This study was undertaken to investigate the factors which may contribute to the strong negative correlation between successive interspike intervals which characteristically occur in the discharge of dorsal spinocerebellar tract (DSCT) neurones.
The findings are in accord with recent studies, suggesting that the fast activity may be conducted in the dorsal spinocerebellar tract and the slower waves in the posterior columns..
The human dorsal spinocerebellar tract (DSCT) was evaluated morphometrically in 14 control cases of different age and sex using semithin sections of epon-embedded cross sections from the C3, T5, and T10 segments of the spinal cord.
The probable pathway is assigned to the dorsal spinocerebellar tract..
This work represents an attempt to elucidate structural features of electrophysiologically characterized, individual cat dorsal spinocerebellar tract (DSCT) neurons by using intracellular application of horseradish peroxidase (HRP).
dorsal spinocerebellar tract (DSCT) neurones adequately activated by primary afferents from the muscle spindles in one muscle in the hindleg of the cat, is regularly inhibited by primary afferents from other muscles.
Examination of the cerebellar system of 5 autopsied patients with X-chromosome-linked copper malabsorption led to the discovery that among cerebellar afferent systems, only the dorsal spinocerebellar tract showed consistent degenerative changes.
Clinical evidence is presented which establishes for the first time that position and vibration sensations may be carried in the dorsal spinocerebellar tracts. The vibratory and position losses were best correlated with the portion of the lesion involving the right dorsal spinocerebellar tract. Arguments are furnished based on physiological experiments in animals that conscious proprioception may be carried by Morin's spinocervicothalamic pathway, which forms part of the dorsal spinocerebellar tract in the spinal cord..
Effects of bulbar reticular tetanic stimulation on the responses of dorsal spinocerebellar tract (DSCT) units were studied in the decerebrate cat.
Responses of dorsal spinocerebellar tract (DSCT) neurons to random electrical stimulation of peripheral nerves of the hindleg in decerebrate cats were studied using cross-correlation analysis of the output spike train.
The repetitive discharge evoked by constant current injection from an intracellular micropipette has been studied in dorsal spinocerebellar tract cells of the cat. It is concluded that the conductance process underlying the postspike afterhyperpolarization is a major factor in the regulation of repetitive firing in dorsal spinocerebellar tract neurones..
The changes in the afterhyperpolarization (a.h.p.) with repetitive activation have been studied in dorsal spinocerebellar tract cells of the cat using intracellular recording techniques.
The longlasting afterhyperpolarization (a.h.p.) following single or short trains of spikes in dorsal spinocerebellar tract (DSCT) neurons of the cat has been studied with intracellular recording techniques.
Ascending axons (the dorsal spinocerebellar tract, DSCT and the spinocervical tract, SCT) did not respond to splanchnic stimuli.
Excitability changes in the dorsal spinocerebellar tract (DSCT) neurons of the cat were extracellularly studied with correlation analysis.
Ten cells were synaptically activated from the ipsilateral cerebellum from the anterior projection zone of the dorsal spinocerebellar tract (DSCT).
activation by axon-collaterals of dorsolateral fascicle (DLF) fibres was observed, indicative of an input from the dorsal spinocerebellar tract (DSCT) to these neurones.
The problem whether the group I hind limb cerebral tract and the dorsal spinocerebellar tract (DSCT) have common spinal axons has been investigated in the present study.
These changes were most prominent in the nuclei of the dorsal spinocerebellar tract.
The assertion made by Burton, Bloedel and Gregory (1971) that neurones in the mLRN (major portion of lateral reticular nucleus comprising its parvi- and magnocellular parts) receive collateral excitation from the dorsal spinocerebellar tract (DSCT) has been tested.
The potential retrograde changes that occur in the proximal segment of transected nerve fibers found in the region of the spinocervical tract (SCT) and in the region of the dorsal spinocerebellar tract (DSCT) after unilateral dorsolateral column (DLC) lesions were studied in kittens and adult cats.
Electrical activity of dorsal spinocerebellar tract (DSCT) was recorded extracellularly and intracellularly in Clarke's column nucleus.
The results allow to assume that the ventral and dorsal spinocerebellar tracts end in the same regions of the cortex of the vermal zone and the intermediate zone of the anterior and posterior cerebellar lobe. The dorsal spinocerebellar tract seems to end on the same side, while the ventral spino-cerebellar tract ends almost exclusively on the opposite side of the symmetry plane..
It is suggested that information carried by dorsal spinocerebellar tract neurones to the cerebellum has a lower signal-to-noise ratio for cutaneous inputs than for muscle or joint inputs..
The relation of the Group I hind limb path to the dorsal spinocerebellar tract is discussed..
Hyperpolarization following single and repetitive excitation of dorsal spinocerebellar tract (DSCT) neurones of the cat was studied by intracellular recording.2.
Information about muscle length is transmitted to the cerebellum from muscle spindle receptors through the dorsal spinocerebellar tract (DSCT).
It was concluded that the path travelled with the dorsal spinocerebellar tract or utilized brain stem collaterals of this tract.6.
The response to muscle stretch of second-order neurons of the dorsal spinocerebellar tract was studied in anesthetized cats.
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