In addition, other structures, including the dorsal prefrontal cortex, amygdala, hippocampus, thalamus, and lateral habenular nucleus, and specific brainstem structures such as the pedunculopontine nucleus, and the raphe nucleus, are key components in regulating the reward circuit. 
Dissociation was seen in the tuberomammillary nucleus where suppression occurred during systemic-induced anesthesia only, and in the lateral habenular nucleus where activity was markedly increased during systemic-induced anesthesia but not following intracerebral microinjection.
The thalamic innervation was very scant and reached the lateral habenular nucleus and the nuclei of the midline.
The reticular nucleus, zona incerta, and lateral habenular nucleus held numerous DAT-ir axons in both species.
Vasopressin-immunoreactive fiber density was sexually dimorphic in the lateral septum, lateral habenular nucleus, medial amygdaloid nucleus, and mediodorsal thalamus. However, deletion of PRs increased the density of vasopressin fiber immunoreactivity in the lateral habenular nucleus.
We investigated the effects of lateral habenular nucleus (LHb) lesions on the behavioral response and on the level of 5-HT in DRN in the depressed rats.
Increased activation of the lateral habenular nucleus leads to the down regulation of the serotonergic, noradrenergic, dopaminergic systems and stimulation of the hypothalamic-pituitary-adrenal (HPA) axis.
We also demonstrated that although amygdaloid input was not as extensive as inputs from other limbic structures such as the medial prefrontal cortex or the lateral habenular nucleus, it was comparable to input from the lateral septal nucleus.
Consistent with the lack of GABA type A signaling as detected by immunohistochemistry, GABA (100 muM) evoked no measurable currents in the medial habenular nucleus but induced bicuculline-sensitive currents in the lateral habenular nucleus and in the CA1 area of hippocampus.
It was found that the right medial habenular nucleus consisted, on average, of 18,000 neurons (with a coefficient of variation of 0.18), while the right lateral habenular nucleus had 13,000 neurons on average (0.14).
We have therefore evaluated, by immunohistochemical image analyses, net alterations of nitric oxide synthases (nNOS, eNOS, iNOS) in brain nuclei [ paraventricular hypothalamic nucleus (PVN), medial habenular nucleus (MHB), lateral habenular nucleus (LHB), paraventricular thalamic nucleus (PV), lateral hypothalamic area (LHA), ventromedial hypothalamic nucleus (VMH), nucleus of the solitary tract (NTS)] of tumor-bearing mice (TB) with prostanoid-related anorexia.
LTC4 synthase immunoreactivity was also observed in the axons of the extrahypothalamic system including the bed nucleus of the stria terminalis, lateral habenular nucleus, midbrain central gray, medial amygdaloid nucleus and ventral septal area, although this immunoreactivity was relatively minor.
Two distinct intrinsic circuits exist in the medial habenular nucleus, whereas in the lateral habenular nucleus, intrinsic axons travel largely from medial to lateral direction.
In the lateral habenular nucleus (LHAB) a similar effect has been found in the control animals re-exposed to the testing cage only (C1 group), and in the MS group, suggesting that this brain area participates predominantly in processing of emotional-cognitive component of a painful stimulation.
Vimentin immunoreactivity was found to be highest at clonus 3 and then decreased at clonus 5 in the hippocampal formation, regions around the third ventricle, caudate putamen and lateral habenular nucleus.
In the 1 h stress group, more FLI neurons were found in the lateral habenular nucleus, the medial habenular nucleus, the paraventricular nucleus, the central nucleus of amgydaloid and the lateral hypothalamus compared with the control group.
administration of ADM (1 nmol/kg, 3 nmol/kg), Fos-like immunoreactivity neurons were markedly increased in several brain areas of the rat, including the nucleus of the solitary tract (NTS), the area postrema, the locus coeruleus, the parabrachial nucleus and the nucleus paragigantocelluaris laterialis (PGL) in the brainstem, the paraventricular nucleus (PVN), the supraoptic nucleus (SON) and the ventromedial hypothalamic nucleus in the hypothalamus, as well as the central amygdaloid nucleus and the lateral habenular nucleus in the forebrain.
Tg mice exhibited significant increases in the total number of neurons in the cerebral cortex (27%), caudate-putamen (27%), dentate gyrus (69%), medial habenular nucleus (61%) and lateral habenular nucleus (36%).
To elucidate the effect of traumatic stress on the lateral habenular nucleus, we investigated the time course of the expression of c-Fos protein in this nucleus of the Japanese monkey (Macaca fuscata) after enucleation of one eye using c-Fos protein immunocytochemistry. c-Fos protein-like immunoreactive neurons were significantly increased; the increase started 1 h after the enucleation and remained high for 3-9 h in the lateral habenular nucleus on both sides. These results suggest that the prolonged expression of c-Fos protein occurred in the lateral habenular nucleus after traumatic stress through multiple transsynaptic activations..
By using extracellular and intracellular recordings in slice preparations, we demonstrate that the activation of synaptic input from the limbic forebrain generates transient hyperpolarizing postsynaptic potentials in neurons of the medial part of the lateral habenular nucleus of the epithalamus.
However, we also found labelled terminals in several other brain areas, including the zona incerta, the medial geniculate nucleus, the lateral posterior-pulvinar complex, the lateral habenular nucleus, and the anterior and lateral hypothalamic regions.
The expression was particularly prominent in the medial habenular nucleus, whereas the lateral habenular nucleus exhibited a lower number of labeled cells.
Other retrogradely labeled neurons were found ipsilateral to the injection site, in the pretectum, the ventral tegmentum, the dorsal nucleus of the posterior commissure and the lateral habenular nucleus.
It was found that c-Fos protein was expressed in neurons of the lateral habenular nucleus, the central nucleus amygdala, the lateral parabrachial nucleus in the pons, and the complex area of the nucleus of the solitary tract and the dorsal motor nucleus of the vagus nerve in the medulla oblongata.
The less numerous and peripherally located type II neurons had an axon that climbed the rostral thalamic pole, coursed along the stria medullaris, and arborized profusely within the lateral habenular nucleus, which stood out as the most densely innervated pallidal target.
In addition, estrogen treatment increased the density of BDNF-ir fibers in the lateral septum, dorsolateral area of the bed nucleus of the stria terminalis, and lateral habenular nucleus.
Moderately to weakly projected areas were the intermediate and lateral parts of the agranular insular cortex, orbital part of area 12, agranular and dysgranular part of the temporal pole cortex (TPa-g), auditory temporal cortex, lateral and medial (MS) septal nuclei, bed nucleus of the stria terminalis, diagonal band of Broca, substantia innominata, and medial preoptic area, dorsomedial, lateral, and posterior hypothalamic nuclei, magnocellular lateral basal and lateral amygdaloid nuclei, paratenial, paraventricular (PV), inter-antero-medial (IAM), reticular, central medial (CeM), parafascicular (PF) and limitans nuclei of the thalamus, lateral habenular nucleus, pedunculo-pontine nucleus, dorsal part of the lateral lemniscal nucleus, ventral tegmental area (VTA), dorsal raphe, superior central nucleus, medial and lateral parabrachial nuclei (PBl) and nucleus locus coeruleus (LC).
In contrast, within the bed nucleus of the stria terminalis, as well as the medial and lateral habenular nucleus of thalamus, immunoreactivity for both proteins was weak.
FGFR4 is reported to be strongly expressed only during early stages of development, and apart from one small region (the lateral habenular nucleus) is not detectable in adult CNS.
The source of the effective forebrain excitation was investigated using electrolytic lesions of documented sources of excitatory amino acidergic afferents to the ventral tegmental area: the medial prefrontal cortex, certain nuclei of the amygdalar complex and the lateral habenular nucleus. Furthermore, this activation appears to be produced by the action of excitatory (probably excitatory amino acidergic) afferents arising from the medial prefrontal cortex, and possibly the lateral habenular nucleus.
Altered c-Fos expression as a consequence of OFQ injection was observed in the nucleus of the solitary tract, paraventricular nucleus of the hypothalamus, supraoptic nucleus, central nucleus of amygdala, lateral septal nucleus and lateral habenular nucleus.
Particularly prominent EC-SOD staining was observed in neurons of the hilar region of the hippocampus, the lateral habenular nucleus of the thalamus, and the suprachiasmatic nuclei of the hypothalamus.
Three regions displayed significant decreases: mammillary body (-34%), anteroventral thalamic nucleus (-28%), and the lateral habenular nucleus (-24%).
AIM: To study effects of sodium L-glutamate microinjection into lateral habenular nucleus (LHN) of rats on the firing of medullary tail-flick related neurons and tail-flick reflex (TF).
Our research has demonstrated that the lateral habenular nucleus (Lhb) is necessary for the hormonal onset but not the postpartum maintenance of maternal behavior in the rat (K.
Wheat germ agglutinin conjugated horseradish peroxidase (WGA-HRP) injection into the midbrain periaqueductal gray (PAG) resulted in heavy accumulation of retrogradely labeled neurons in the lateral habenular nucleus (LHb) bilaterally.
Both AMPA and NMDA markedly increased glucose use in the striatum and globus pallidus, with concomitant perturbations in striato-pallidal projection targets including the substantia nigra, entopeduncular nucleus, subthalamic nucleus and lateral habenular nucleus.
Fibers for both AVP and OT were evident outside of the hypothalamic-neurohypophyseal tract, but a plexus of AVP-ir fibers in the lateral septum or lateral habenular nucleus, as seen in the rat brain, could not be detected for either peptide..
After raphe magnus or gigantocellular reticular pars alpha injections, a moderate to substantial number of cells were localized in the fields of Forel, lateral habenular nucleus and ventral caudal pontine reticular nucleus.
MARCKS hybridization was most pronounced in the olfactory bulb, piriform cortex (layer II), medial habenular nucleus, subregions of the amygdala, specific hypothalamic nuclei, hippocampal granule cells, neocortex, and cerebellar cortex, intermediate in the superior colliculus, hippocampal CA1, and certain brainstem nuclei including the locus coeruleus, and low-absent in regions of the caudate-putamen, geniculate, thalamic nuclei, lateral habenular nucleus, and hippocampal CA3 pyramidal and hilar neurons. Consistent with previous reports, prominent F1/GAP-43 hybridization was observed in neocortex, medial geniculate, piriform cortex (layer II), substantia nigra pars compacta, hippocampal CA3 pyramidal cells, thalamic and hypothalamic nuclei, lateral habenular nucleus, locus coeruleus, raphe nuclei, and cerebellar granule cells, intermediate in regions of the thalamus, hypothalamus, and amygdala, and low-absent in regions of the olfactory bulb, caudate-putamen, medial habenular nucleus, hippocampal granule cells, and superior colliculus.
Labeling was also found in the anterior medial preoptic nucleus and, in relatively sparse amounts, in the lateral geniculate nucleus, superior colliculus and lateral habenular nucleus.
The lateral habenular nucleus is shorter and less demarcated than hm.
Treatment with 1 mg/kg muscimol led to further significant decreases of Fos expression in CA1-3 pyramidal neurons and the disappearance of Fos induction in the cerebral cortex above the rhinal fissure, reticular thalamic nucleus, claustrum, fundus striati, ventral pallidum, septal nucleus, lateral habenular nucleus, and lateral amygdaloid nucleus.
Especially abundant fiber- and terminal-like patterns were localized to superficial layers of the spinal cord dorsal horn and nucleus caudalis of the spinal tract of the trigeminal, the nucleus of the solitary tract, nucleus ambiguous, locus coeruleus, interpeduncular nucleus, medial aspect of the lateral habenular nucleus, presumed "striasomes" of the caudate-putamen and nucleus accumbens.
When lateral habenular nucleus (LHb) was stimulated the on-cells were excited, the off-cells inhibited while the neutral cells unaffected.
Following separate unilateral injections of Fluoro-Gold (FG), Fluoro-Ruby (FR), or 4-acetamido,4- isothiocyanostilbene-2,2'-disulfonic acid (SITS) into the lateral habenular nucleus (LHB), a small population of ganglion cells was labeled sporadically, predominantly those in the nasal retina contralateral to each injection site.
At 0.5 microgram and 1 microgram intracerebroventricularly the highly selective mu-opioid receptor agonist D-Ala2, MePhe4, Gly-ol5-enkephalin effected statistically significant increases as well as statistically significant decreases in regional glucose utilization: in limbic structures, such as hippocampal formation, medial amygdala and lateral septum, glucose utilization was most prominently increased after D-Ala2, MePhe4, Gly-ol5-enkephalin; glucose utilization was further increased in the lateral habenular nucleus, the hypothalamus, ventromedial nucleus and dorsal raphe; whereas decreases were found in the mamillary body and anterior thalamus.
Significant metabolic changes among the four groups were found in five brain regions, including the nucleus accumbens and the lateral habenular nucleus.
L-Glutamic acid (L-Glu) was microinjected into medial (MHb) and lateral habenular nucleus (LHb) on light anesthetized or conscious rats, and pain threshold was then determined on the different models of animal.
A very large population of neurotensin-containing cell bodies was observed in the septal nuclei, nucleus accumbens septi, preoptic areas, bed nucleus of the stria terminalis, olfactory tubercle, entorhinal cortex, ventral subiculum, anterodorsal thalamic nucleus, anteroventral thalamic nucleus, nucleus reuniens, lateral habenular nucleus, parabrachial nucleus, and nucleus of the solitary tract.
The labeled cells were found in the medial part of lateral habenular nucleus, posterior hypothalamic area, lateral subnucleus of interpeduncular nucleus, dorsal raphe nucleus, periaqueductal gray matter, dorsal paramedial nucleus, preposital hypoglossal nucleus, dorsal paragiganto-cellular nucleus, magnum raphe nucleus, and fastigatum of cerebellum.
Immunoreactive nerve fibers were observed in the main and accessory olfactory bulbs, olfactory tubercle, prelimbic area, anterior cingulate cortex, neostriatum, accumbens, lateral septal nucleus, lateral habenular nucleus, and superior colliculus.
The density of vasopressin-immunoreactive (AVP-ir) fibers in the lateral septum and lateral habenular nucleus is lower in prairie vole fathers--which display paternal behavior under natural conditions-than in sexually naive males. To see if these changes occur before or after the birth of pups, and whether they are related to changes in paternal behavior, we tested paternal responsiveness and measured AVP-ir fiber density in the lateral septum, lateral habenular nucleus, medial preoptic area, and paraventricular nucleus of the thalamus of sexually naive males and females (0P) and breeding pairs that were sacrificed shortly after mating (3P); during early (13P); or late gestation (21P); or after the birth of pups (6PP). The fiber density in the lateral septum and lateral habenular nucleus was affected differently in males and females.
Three days of male and female cohabitation dramatically reduces the density of vasopressin-immunoreactive (AVP-ir) fibers in the lateral septum and lateral habenular nucleus of male, but not of female prairie voles. The previously observed decrease in AVP-ir fiber density in the lateral septum and lateral habenular nucleus may therefore reflect increased synaptic release of AVP, which may contribute to mating-induced changes in social behaviors in prairie vole males..
Specific hybridization to the vasopressin V1aR mRNA was evident in cells of the frontal cortex, piriform cortex, internal granular layer and the medial, dorsal, ventral and lateral portion of the anterior olfactory nucleus, zona limitans of the islands of Calleja, suprachiasmatic nucleus, CA1, CA2, CA3 and dentate gyrus of the hippocampus, paraventricular hypothalamic nucleus, ventromedial hypothalamic nucleus, arcuate nucleus, lateral habenular nucleus, and the molecular and granular cell layers of the cerebellum.
Additionally, a series of fluorescent retrograde double-labeling experiments with Fast Blue and Diamidino Yellow have indicated that single entopeduncular nucleus neurons projecting to the superior colliculus or lateral tegmental field often send their axon collaterals to the lateral habenular nucleus.
administration of CI-977 (0.03-3 mg/kg) induced relatively homogeneous patterns of altered cerebral glucose utilization with moderate statistically significant reductions (approximately 25%) being observed in 29 brain regions, and a statistically significant increase (approximately 40%) in one brain region, the lateral habenular nucleus.
The low dose did not produce significantly altered glucose metabolism in the nucleus accumbens or in the lateral habenular nucleus as observed with most other neuroleptics, suggesting a lack of antipsychotic action at this dose.
For instance, the interpeduncular nucleus, the lateral habenular nucleus and the deep layers of the cerebral cortex were labeled in the guinea pig but not in the rat while the reverse was observed for the columns of the vermis lobules 9-10, the dorsal raphe nucleus, the medial habenular nucleus, the superficial cortical layers and the dorsal hippocampus.
Stimulation induced a decrease in two regions of the primary auditory system and in the lateral habenular nucleus.
The lateral habenular nucleus of the rat contains a dense plexus of dopaminergic fibers, which are more marked in the medial part of the lateral habenular nucleus than in its lateral counterpart. Employing a combination of fluorescent retrograde axonal tracing with fluorogold and tyrosine hydroxylase immunofluorescence histochemistry, we investigated the distribution of cells of origin of the dopaminergic afferent fibers to the lateral habenular nucleus in the rat. The cells double-labeled with both fluorogold injected into the lateral habenular nucleus and tyrosine hydroxylase antisera were seen in a variety of fore- and midbrain regions, including the bed nucleus of the stria terminalis, medial preoptic area, periventricular, ventromedial, and dorsomedial hypothalamic nuclei, ventral tegmental area, interfascicular nucleus, substantia nigra pars compacta, ventrolateral division of the midbrain periaqueductal gray, and dorsal raphe nucleus. We have further observed by anterograde axonal tracing with Phaseolus vulgaris-leucoagglutinin that projection fibers arising from the sites of origin of the dopaminergic afferent fibers to the lateral habenular nucleus terminate mainly in the medial part of the lateral habenular nucleus, and to a lesser extent in its lateral counterpart. Thus, we have found in the present study that the dopaminergic neurons sending their axons to the lateral habenular nucleus are widely distributed in the A9, A10, A14, and A15 dopaminergic cell groups. Such dopaminergic neurons may exert regulatory influences upon many limbic-associated brain regions via the lateral habenular nucleus..
For both species, the densities of AVP-immunoreactive (AVP-ir) fibers in the lateral septum, lateral habenular nucleus, medial preoptic area and paraventricular nucleus of the thalamus were compared in males and females that were sexually inexperienced or had become parents 6 days before sacrifice. The lateral septum and lateral habenular nucleus presumably receive their projections from the bed nucleus of the stria terminalis and medial amygdaloid nucleus, while the other two areas presumably receive their projections from the suprachiasmatic nucleus. In both species, AVP-ir fiber densities in the lateral habenular nucleus and the lateral septum were much greater in males than in females regardless of parental state. In prairie voles, AVP-ir fiber density in the lateral septum and lateral habenular nucleus was reduced in parental males, while no differences were found in females. In parental meadow voles, the AVP-ir fiber density in the lateral septum did not show changes, while the fiber density in the lateral habenular nucleus was increased..
Persistent noxious peripheral stimulation by formalin injection into the unilateral hindpad of anaesthetized rats induced c-Fos-like protein immunoreactivity (c-Fos-LI) in neurons within the lateral habenular nucleus (LHb) bilaterally.
The descending efferent projection from the ventral pallidum in monkeys terminates primarily in the subthalamic nucleus and adjacent lateral hypothalamus, in the substantia nigra, and in the lateral habenular nucleus.
Buspirone at 1 and 10 mg/kg increased glucose utilization by 40% and 65%, respectively, in the lateral habenular nucleus, whilst 1-PP at 1 and 10 mg/kg had no effect.
But it was further found that either amphetamine or cocaine given continuously over a 3- to 5-day period induce a highly specific pattern of axonal degeneration extending from the lateral habenular nucleus along the fasciculus retroflexus towards the ventral tegmentum.
Immunocytochemical studies using the PAP method revealed that tyrosine hydroxylase- and L-DOPA positive but aromatic L-amino acid decarboxylase- and DA-negative neurons were present in the lateral habenular nucleus of the house-shrew (Suncus murinus) brain.
The most pronounced increases were seen in the lateral habenular nucleus (331 +/- 30% of control), the zona incerta (400 +/- 55%), the mesencephalic reticular formation (415 +/- 122%) and the parietal cortex (211 +/- 35%).
In control rats, BMY 7378 (5 mg/kg, s.c.) markedly increased glucose utilization in the lateral habenular nucleus and moderately reduced glucose utilization in the hippocampal formation.
Dopamine (DA) and noradrenaline (NA) extracellular levels have been measured by microdialysis in the medial frontal cortex (MFC), nucleus accumbens (NAc) and caudate-putamen (CP) under baseline conditions in awake and halothane-anaesthetized rats, and after application of three types of stimuli which are likely to activate the brainstem catecholaminergic systems: mild stressors (handling and tail pinch), rewarded behavior (eating palatable food without prior food deprivation) and electrical stimulation of the lateral habenular nucleus.
Both OXT and VP fibers were distributed in the lateral habenular nucleus, stria medullaris thalami, lateral preoptic area, stria terminalis, and medial and supracapsular part of the bed nucleus of the stria terminalis.
The lateral habenular nucleus is innervated by various fiber groups originating from the bed nucleus of the anterior commissure, the diagonal band nucleus, the lateral preoptic area, the anterior entopeduncular nucleus, the lateral hypothalamic and mammillary areas, the nucleus of the stria medullaris, the area tegmentalis ventralis and a scattered neuronal subpopulation in the large-celled dorsolateral nucleus of the dorsal thalamus.
All three 5-HT1A partial agonists reduced glucose utilization in the hippocampus and dentate gyrus by 20-25% and increased glucose utilization by 38-65% in the lateral habenular nucleus; an important relay between striatal/limbic areas and the mid-brain raphe nuclei.
After a further 30 min, a massive bilateral induction of fos was observed in the lateral habenular nucleus (LHb), the PV, the rhomboid thalamic nucleus, and the intralaminar nuclei of the thalamus.
In the left ganglion seen at the level of the rostrocaudal middle, the dorsal bundle gave off collaterals to the lateral habenular nucleus (LH) and dorsal subnucleus of the medial habenular nucleus (MH), while the ventral bundle innervated the intermediate and ventral subnuclei of the MH.
The HG was made up of the medial (MH) and lateral habenular nucleus (LH), and the former nucleus was further divided into a dorsal, intermediate and ventral subnucleus.
Computer maps revealed high values in the outer and inner layers of the cortex, some hippocampal and olfactory bulb layers, prepiriform cortex, dorsal part of the caudate-putamen, globus pallidus, lateral septum, reticular thalamic nucleus, lateral habenular nucleus, circumventricular organs, nuclei of the medial hypothalamus, substantia nigra, interpeduncular nucleus, and mamillary body.
In non-hibernating animals, Met-EK-immunoreactive perikarya were observed in telencephalic (putamen, caudate nucleus, medial septum-diagonal band complex, amygdala) and diencephalic (periventricular hypothalamic nucleus, lateral hypothalamic area) regions, whereas immunoreactive fibers were found in the lateral septum, stria terminalis nucleus, various hypothalamic areas, arcuate nucleus, median eminence, thalamic intralaminar, periventricular nucleus and lateral habenular nucleus.
Electrical stimulation of the lateral habenular nucleus (15 Hz, 0.5 mA) in halothane-anaesthetized rats induced a significant increase in NA output both in the intact and grafted hippocampi.
In the diencephalon, neurons were labeled in the midline nuclear complex of the thalamus, the lateral habenular nucleus, and the hypothalamus.
In stimulated animals, the KROX-24-IR was induced in many areas including the lateral reticular nucleus, parabrachial nucleus, periaqueductal gray, hypothalamus, amygdala and lateral habenular nucleus..
Immunocytochemistry showed that males had significantly more AVP-immunoreactive (AVP-IR) cells in the BST and significantly denser AVP-IR projections from this nucleus to the lateral septum, lateral habenular nucleus, and periaqueductal central gray than did females.
Other areas with a substantial number of VTi fibers are the lateral habenular nucleus, the ventral tegmental area, the substantia nigra, the locus coeruleus and the nucleus of the solitary tract. Sex-related differences in the density of the VTi fibers were observed in the lateral septal nucleus, the mid-brain periaqueductal gray and, to a lesser extent, in the ventral amygdaloid nucleus, the lateral habenular nucleus, the ventral tegmental area and the substantia nigra.
In this context, diffuse, non-fibrillar receptor immunoreactivity occurred in the lateral habenular nucleus and medial terminal nucleus of the accessory optic tract.
The data showed that the pain threshold of animal was increased when septal area was stimulated by electro-acupuncture, and that electrical stimulation of septal area had a marked inhibitory effect on the pain discharges of cells in parafascicular nucleus of thalamus, lateral habenular nucleus, periaqueductal gray and dorsal raphe nucleus.
The common efferent projections were in the subthalamic nucleus, lateral habenular nucleus, pretectal nucleus, posterior commisure nucleus, nucleus of Darkschewitsch, pontine nucleus, nucleus reticularis tegmenti pontis, medial accessory inferior olive, and the superior colliculus.
Therefore it is suggested that double-labeled neurons in the entopeduncular nucleus project to the lateral habenular nucleus which is involved in the limbic system, since the rostral portion of the entopeduncular nucleus has been shown to project to the lateral habenular nucleus..
Studies of the distribution of I-MEL binding in rat, Syrian hamster, and Djungarian hamster brain confirm that the median eminence and suprachiasmatic nucleus are major sites of I-MEL binding in rodent brain; other brain areas labeled in one or more of these species were the thalamus (paraventricular, anteroventral, and reuniens nuclei, nucleus of the stria medullaris, and medial part of the lateral habenular nucleus), hypothalamus (dorsomedial nucleus), subiculum, and area postrema.
At anterior levels the putative inverted sublobule of the LGV merges imperceptibly with the neuroepithelium that produces the neurons of the lateral habenular nucleus.
Field potential studies in vivo have shown that many subcortical structures, such as the inferior olivary nucleus, the thalamic nuclei and the lateral habenular nucleus, generate behaviorally relevant rhythms.
Since the rostral portion of the entopeduncular nucleus mainly projects to the lateral habenular nucleus, it is suggested that double-labeled neurons in the entopeduncular nucleus largely project to the lateral habenular nucleus which is involved in the limbic system..
Based upon retrograde HRP labeling, the principal afferents to the VTN region of the cmPRF originated from the medial and lateral mammillary nuclei, and lateral habenular nucleus, and to a lesser extent from the interpeduncular nucleus, lateral hypothalamus, dorsal tegmental nucleus, superior central nucleus, and contralateral nucleus reticularis pontis caudalis.
The regional changes in glucose utilization induced by U-50,488H in the brain were most pronounced in components of the limbic forebrain circuit such as the anterior thalamic nuclei, mammillary body, frontal cortex, lateral septal nucleus, nucleus accumbens and lateral habenular nucleus as well as in the brainstem tegmental nuclei and the dorsal and median raphe nucleus (components of the limbic midbrain area).
Some brain areas relating to pain, for instance, locus coeruleus, raphe nuclei, periaqueductal gray, hypothalamus, lateral habenular nucleus, amygdaloid complex, hippocampus and cingulate cortex, project to medial septal nucleus, lateral nucleus and diagonal band nucleus.
Pallidal terminals in the cat lateral habenular nucleus and the ventral anterior and ventral lateral nuclear complex of the thalamus have been examined electron microscopically.
During postnatal development of normal mice dense networks of neurophysin II immunoreactivity in the lateral septal nucleus and lateral habenular nucleus appeared earlier in male than in female mice, with an adult pattern of immunoreactivity being attained by 8 weeks and 12 weeks of age, respectively.
A small retinal projection to the lateral habenular nucleus has not been described previously.
Similar stimulation of the globus pallidus increased LGCU in the globus pallidus, substantia nigra pars reticulata and compacta, entopeduncular nucleus, subthalamic nucleus, lateral habenular nucleus, parafascicular nucleus of the thalamus, deep layers of the superior colliculus and pedunculopontine nucleus, exclusively on the ipsilateral side.
The efferent connections of the rat lateral habenular nucleus (LHb) were demonstrated using anterograde transport of the lectin Phaseolus vulgaris leucoagglutinin (PHA-L).
Small to moderate numbers of immunoreactive fibers were present in the lateral habenular nucleus, ventral lateral geniculate nucleus, zona incerta, parataenial, mediodorsal, medioventral, and submedial nuclei, the rhomboid, paracentral, central lateral and parafascicular nuclei, and in the medial geniculate and dorsal lateral geniculate nuclei.
The inhibitory response evoked in presumably serotonergic dorsal raphe neurons by stimulation of the lateral habenular nucleus was examined in the rat using intracellular recording techniques.
Other areas containing SLI included the striatum (caudate nucleus and putamen), zona incerta, infundibulum, supramammillary and premammillary nuclei, medial and dorsal lateral geniculate nuclei, entopeduncular nucleus, lateral habenular nucleus, central medial thalamic nucleus, central tegmental field, linear and dorsal raphe nuclei, nucleus of Darkschewitsch, superior and inferior colliculi, nucleus ruber, substantia nigra, mesencephalic nucleus of V, inferior olivary nucleus, inferior central nucleus, nucleus prepositus, and deep cerebellar nuclei.
Following True blue (TB) injections into the CPU and Diamidino yellow (DY) injections into the lateral habenular nucleus (lHb), all the TB-positive neurons in the rostral 1/3 of the Ep were unequivocally double-labeled with DY.
In the habenula, a few GABA-immunoreactive cell bodies and numerous GABA-positive terminals were scattered throughout the lateral habenular nucleus, whereas only a few GABA-immunoreactive terminals surrounded the closely packed unreactive cells in the medial habenular nucleus.
Leucine-enkephalin-like immunoreactive (L-ENKI) fibers were observed in the dorsal portion of the medial habenular nucleus (MHb), the intermediate portion of the lateral habenular nucleus (LHb), and the border zone between the MHb and the LHb (BZHb).
In contrast to the unanimous decrease of LCBF in the ipsilateral cortices and the lateral striatum, complexed changes in LCGU were found in not only the cortex and striatum but also in many other subcortical regions which were closely related to the distribution of the mesencephalic dopamine neurons, such as globus pallidus, substantia nigra, subthalamic nucleus, nucleus accumbens, olfactory tubercle and lateral habenular nucleus.
Different branches of this system innervate the midbrain (superior colliculus, interstitial magnocellular nucleus of the posterior commissure, and anterior pretectal nucleus), the diencephalon (lateral habenular nucleus, parafascicular, anteroventral, anterodorsal, mediodorsal, and intralaminar thalamic nuclei), and the telencephalon (lateral septum and medial prefrontal cortex). Retrogradely labelled cells were also located bilaterally in the premammillary nucleus, paraventricular hypothalamic nucleus, zona incerta, and lateral habenular nucleus. The projections of the medial prefrontal cortex, the bed nucleus of the stria terminalis, and the lateral habenular nucleus to the TLD were confirmed in anterograde tracing studies. The latter include the lateral habenular nucleus and medial prefrontal cortex..
L-DOPA decreased RCGU in the ipsilateral lateral habenular nucleus (LHN) and increased RCGU in the contralateral LHN, changes that we suggest are mediated via altered neuronal activity in the striatum and EP.
Positive fibers were found in the thalamus in and around the periventricular nucleus as well as in the lateral habenular nucleus and extending in a lateral, caudal direction from the third ventricle and fasciculus retroflexus to the lateral tip of the medial lemniscus.
Large numbers of labeled cells appeared in the lateral hypothalamus, lateral habenular nucleus, ventral tegmental area, periaqueductal gray, parabrachial nuclei and nucleus raphe magnus. The most prominent aggregation of D-[ 3H]Asp-labeled cells was found in the lateral habenular nucleus, indicating that this input operates with an excitatory amino acid as transmitter.
A marked decrease of AVP fiber density was found in the old rats as compared with the young animals in the vertical limb of the diagonal band, the basal nucleus of Meynert, the lateral habenular nucleus, the medial amygdaloid nucleus, the substantia nigra, the ventral hippocampus, the central gray, the locus coeruleus and in the ambiguus nucleus.
The TVP receives many fibers from the medial division of the ipsilateral medial mammillary nucleus and bilaterally from the lateral habenular nucleus, and additionally some fibers from the posterior nucleus of the interpeduncular complex. The TDV receives many fibers from the ipsilateral lateral mammillary nucleus, from the ipsilateral prepositus hypoglossi nucleus, bilaterally from the lateral habenular nucleus, from the central and paramedian nuclei of the interpeduncular complex, from the bilateral gray matter along the floor of the fourth ventricle, and from the contralateral supragenual nucleus. The TDD receives a projection from the lateral habenular nucleus of both sides and from the central and paramedian nuclei of the interpeduncular complex, and a minor projection from the ipsilateral lateral mammillary nucleus, the posterior nucleus of the interpeduncular complex, the prepositus hypoglossi nucleus, and the contralateral supragenual nucleus..
Furthermore, a very strong reduction was seen in the density of vasopressin-immunoreactive fibers in the olfactory tubercle, nucleus of the diagonal band and its immediate surroundings, ventral pallidum, basal nucleus of Meynert, lateral septum, septofimbrial nucleus, ventral hippocampal formation, amygdaloid area, pre- and supramammillary nucleus, supramammillary decussation, (inter)dorsomedial, parafascicular, and ventral aspect of paraventricular thalamic nuclei, zona incerta, lateral habenular nucleus, ventral tegmental area, substantia nigra, periventricular gray, dorsal and median raphe nucleus, and locus coeruleus.
The areas which had only efferent connections from MP were accumbens, caudate putamen, ventral pallidum, substantia innominata, lateral habenular nucleus, paratenial thalamic nucleus, paraventricular thalamic nucleus, mediodorsal thalamic nucleus, reuniens thalamic nucleus, median eminence, medial mammillary nucleus, subthalamic nucleus, pars compacta of substantia nigra, oculomotor nucleus, red nucleus, laterodorsal tegmental nucleus, reticular tegmental nucleus, cuneiform nucleus, nucleus locus coeruleus, and dorsal motor nucleus of vagus among which substantia innominata and median eminence were previously reported.
Smaller numbers of afferent fibers arise from the lateral habenular nucleus, the posterior hypothalamus and the brainstem reticular formation.
The DA axons were found to be aggregated in a dense terminal field located in the caudal two thirds of the medial part of the lateral habenular nucleus. This study thus documents the existence of a mesohabenular DA pathway whose cell bodies are located in the ventral mesencephalon and whose axons ascend with the fasciculus retroflexus to terminate in the caudomedial part of the lateral habenular nucleus.
Significant focal alterations in glucose utilization occurred in a number of other regions ipsilateral to the injection, including lateral habenular nucleus (increased by 16%), pars compacta of the substantia nigra (increased by 28%), ventrolateral nucleus of the thalamus (decreased by 40%), sensory-motor cortex (decreased by 47%), deep layer of the superior colliculus (decreased by 18%), and subthalamic nucleus (decreased by 18%).
We examined the distribution and afferent projections of substance P-like immunoreactive (SPI) fibers in the lateral habenular nucleus of the rats by using the indirect immunofluorescence method. On the basis of the distribution, a dense plexus of SPI fibers in the lateral habenular nucleus (LHb) could be divided into two parts: medial (mLHb) and lateral (lLHb).
reuniens of the thalamus and in the lateral habenular nucleus of the epithalamus. Somatostatin fibres are very dense in the lateral habenular nucleus.
Other areas where fibers were seen to decrease after lesions of the bed nucleus are the diagonal band of Broca, the area of the anterior amygdala, the lateral habenular nucleus, the periventricular gray, and the locus coeruleus..
periventricular and paraventricular hypothalamic nuclei, lateral habenular nucleus) regions which were examined. In contrast, glucose utilization in the lateral habenular nucleus was elevated significantly following phenoxybenzamine only in animals bearing unilateral locus coeruleus lesion; the drug being without effect in this region in sham-lesioned rats.
Extracellular recordings were made from single neurons in the lateral habenular nucleus of urethane-anesthetized rats. These electrophysiological findings support the suggestion from recent neuroanatomical studies that the lateral habenular nucleus is a site of integration for entopeduncular and limbic inputs and in turn sends signals to the midbrain..
Significantly increased glucose utilization after intrastriatal injection of vasoactive intestinal polypeptide was observed in a number of regions (e.g., substantia nigra pars compacta, entopeduncular nucleus, lateral habenular nucleus, entorhinal, pyriform cortices, and amygdala) with known primary or secondary neuronal connections with the caudate nucleus.
kg-1) effected significant increased in glucose utilization in 15 regions of the CNS (e.g., subthalamic nucleus, ventral thalamic nucleus, rostral neocortex, substantia nigra, pars reticulata), and significant reductions in glucose utilization in two regions of the CNS (lateral habenular nucleus and anterior cingulate cortex).
Substantial activity also existed in the globus pallidus, the median eminence, the supraoptic and paraventricular nuclei, the lateral habenular nucleus and the organon vasculosum laminae terminalis.
Since after lesioning the vasopressinergic fibers remained unaltered in the lateral septum, the lateral habenular nucleus, the nucleus of the amygdala, the diagonal band of Broca, the nucleus of the solitary tract, the interpeduncular nucleus and the dorsal raphe nucleus, the suprachiasmatic nucleus sends no or only minor projections to these areas.
When the area of the HRP injection involved the midbrain reticular formation adjacent to the IP complex and the nucleus reticularis tegmenti pontis (RT) but not the IP complex itself, there were many labeled cells in the lateral habenular nucleus and the medial and lateral mammillary nuclei, but there were no labeled cells in the medial habenular nucleus..
Hemorrhagic hypotension (mean arterial pressure reduced by approximately 50 mm Hg) effected significant increases in glucose utilization in eight areas of the central nervous system, namely, the nucleus of the tractus solitarius (glucose utilization increased by 38%), the dorsal motor nucleus of the vagus (by 36%), locus coeruleus (by 38%), lateral habenular nucleus (by 40%), periventricular nucleus of the hypothalamus (by 41%), paraventricular nucleus of the hypothalamus (by 97%), supraoptic nucleus (by 86%), and the interstitial nucleus of the stria terminalis (by 84%).
On precipitation of morphine withdrawal by subcutaneous administration of naloxone at 0.5 mg/kg to morphine-dependent rats, glucose utilization was increased in the central nucleus of amygdala (51%), lateral mammillary nucleus (40%), lateral habenular nucleus (39%), medial mammillary nucleus (35%), and medial septal nucleus (35%) (all, P less than 0.01).
Labeled fibers in the stria medullaris project to the lateral habenular nucleus.
Stimulation of the lateral preoptic region or of the anterior half of lateral hypothalamus produced activation of the lateral septal nucleus, lateral habenular nucleus, perifornical region, midline thalamus and ventral tegmental area.
These structures were: substantia nigra, cerebral cortex, ventromedial and center median-parafascicular thalamic nuclei, nucleus accumbens and posterior hypothalamic areas, including the medial forebrain bundle and lateral habenular nucleus.
At gestational day 20, SRIF-positive cells newly appear in the septum, olfactory bulb, diagonal band of Broca, claustrum, lateral preoptic area, and lateral habenular nucleus.
After HRP injections into the lateral hypothalamic area, labeled cells were found mainly in the medial prefrontal and infralimbic cortices, lateral and dorsal septal nuclei, nucleus accumbens, bed nucleus of the stria terminalis, medial and lateral amygdaloid nuclei, lateral habenular nucleus, peripeduncular nucleus, ventral tegmental area, mesencephalic and pontine central gray, ventral nucleus of the lateral lemniscus, lateral parabrachial area, raphe nuclei and the nucleus locus coeruleus.
The animals learned to bar press for electrical stimulation of the medial or lateral habenular nucleus or the fasciculus retroflexus, but not the surrounding thalamic nuclei.
Although fibers appeared on the periventricular nucleus already on the 7th postnatal day, such fibers were visible in the lateral septum and lateral habenular nucleus only on day 10. From the 12th postnatal day onwards a marked sex difference developed with respect to the density of the vasopressin fibers in the lateral septum and, to a lesser extent, in the lateral habenular nucleus.
The organization of afferent projections to the lateral habenular nucleus (LHB) was studied in adult cats.
In addition, glucose utilization in ipsilateral lateral habenular nucleus was increased at each of the above time points. These results suggest that lesion of the substantia nigra with depletion of striatal dopamine content results in disinhibition of some striatal, and perhaps olfactory cortical, efferents producing increased metabolism and glucose utilization in terminal fields within the globus pallidus and lateral habenular nucleus..
In the lateral habenular nucleus three neuronal types were found, all of them with long, poorly ramified dendrites, but differing with respect to the size of their somata and their dendritic profiles.
(b) Recordings were made from 42 units in the lateral habenular nucleus.
It was possible to observe excitation in 18 units following electrical stimulation of the lateral habenular nucleus, and of these cells, nine units were additionally tested with ACH.
Concurrently, structures receiving projections from the neostriatum (globus pallidus, entopeduncular nucleus, and substantia nigra, pars reticulata) show enhanced labeling in the 6-hydroxydopamine-injected hemisphere, as do diencephalic structures receiving basal ganglia inputs (ventromedial nucleus and ventrolateral portion of the ventral anterolateral complex of thalamus, lateral portion of lateral habenular nucleus).
The alterations in glucose utilization in the lateral habenular nucleus following the systemic administration of a putative dopaminergic agonist and antagonist have been examined in 48 rats by means of the autoradiographic 2-deoxyglucose technique.
Fibers arising from the bed nucleus of the anterior commissure are distributed to the preoptic region, lateral hypothalamus, supramammillary region, posterior aspect of the medial mammillary nucleus and lateral habenular nucleus..
From the parvocellular suprachiasmatic nucleus only vasopressin containing fibers were found to run towards the organum vasculosum lamina terminalis, lateral septum, and lateral habenular nucleus. Immunoelectron microscopy, using the preembedding staining technique, frequently demonstrated vasopressin-containing synapses on neuronal structures in the lateral septum, lateral habenular nucleus, and amygdala.
Following lesions in the lateral hypothalamic complex (LHC) which produce aphagia and adipsia as well as the injection of labeled proline into the LHC, projections were seen in the nucleus accumbens, the preoptic area, the lateral habenular nucleus, the ventromedial hypothalamic nucleus, the nucleus reuniens, the parafascicular nucleus, the posterior hypothalamus, the zona incerta, the central gray matter, the tegmentum, the substantia nigra (pars compacta), the ventral tegmental area of Tsai and the parabrachial area.
The ascending components of the MFB originate in: (1) the medial preoptic nucleus, (2) the nucleus periventricularis stellatocellularis and rotundocellularis, (3) the posterior hypothalamic nucleus, (4) the parafascicular nucleus, (5) the ventral premammillary nucleus, (6) the substantia grisea periventricularis, (7) the lateral habenular nucleus, (8) the VM and DM, (9) the paratenial nucleus, (10) the AM and (11) the arcuate nucleus..
By contrast, enhanced uptake was found in the ipsilateral globus pallidus, entopeduncular nucleus, the area lateral to the lateral hypothalamus, the lateral habenular nucleus and pars reticulata of the substantia nigra.
The differentiating, radioresistant neurons of the lateral habenular nucleus, derived from a portion of the superior neuroepithelial lobule (SL1), were settling by day E15 and by this time the habenulopeduncular tract was forming.
The neurons of the lateral habenular nucleus are produced between days E13--16.
Small injections of tritiated leucine and proline confined to the ventral tegmental area (AVT) were found to label fibers ascending: (a) to the entire ventromedial half of the striatum, but most massively to the ventral striatal zone that includes the nucleus accumbens; (b) to the thalamus: lateral habenular nucleus, nuclei reuniens and centralis medius, and the most medial zone of the mediodorsal nucleus; (c) to the posterior hypothalamic nucleus and possibly the lateral hypothalamic and preoptic region; (d) to the nuclei amygdalae centralis, lateralis and medialis; (e) to the bed nucleus of the stria terminalis, the nucleus of the diagonal band, and the medial half of the lateral septal nucleus; (f) to the anteromedial (frontocingulate) cortex; and (g) to the entorhinal area.
EPN axons also terminated in the rostral portion of the centrum medianum (CM), the ventrolateral portion of the lateral habenular nucleus (LHB), and the pedunculopontine nucleus (PP).
Thus, the anterior and lateral parts of the LHA also appear to project substantially to the anterior hypothalamic area, the ventromedial and dorsomedial hypothalamic nuclei, the parataenial and paraventricular nuclei of the thalamus, and the medial part of the lateral habenular nucleus.
Pallidal efferents are also distributed to a still obscure tegmental area in the midbrain, the pedunculopontine nucleus, and to the lateral habenular nucleus, a structure of the limbic system. Only 25% of entopeduncular neurons responded antidromically to stimulation of the lateral habenular nucleus exclusively (one-third) or not (two-thirds). Neurons were also recorded in the preoptico-hypothalamic area and 67% responded antidromically exclusively to the stimulation of the lateral habenular nucleus..
In addition, a vasopressin containing pathway between the suprachiasmatic nucleus and the lateral habenular nucleus was demonstrated.
The lateral habenular nucleus receives a substance P projection from the medial habenular nucleus and is the source of cholinergic projection to the interpeduncular nucleus and to the medial habenular nucleus.
One of the most striking differences is that the entopeduncular nucleus of the cat contains very few HRP labelled cells after lateral habenular nucleus injection in comparison to the entopeduncular nucleus of the rat which can be shown to be the major source of afferents to the lateral habenular nucleus by the same method..
Fibers from the ventral half of the septum (i.e., nucleus of the diagonal band) project to medial and lateral sectors of the hypothalamus, dorsomedial thalamus, lateral habenular nucleus and midbrain tegmentum.
Fibers from the ventral half of the septum (i.e., nucleus of the diagonal band) project to medial and lateral sectors of the hypothalamus, dorsomedial thalamus, lateral habenular nucleus and midbrain tegmentum.
The present results indicate that the NRD, particularly is rostral part, receives direct projections arising from: (1) locus coeruleus complex (locus coeruleus, locus coerulus alpha, and locus subcoeruleus); (2) parabrachial nuclei (nucleus parabrachialis lateralis and medialis); (3) nucleus laterodorsalis tegmenti; (4) griseum centrale pontis, particularly the caudal part of the nucleus incertus; (5) substantia nigra; (6) lateral habenular nucleus; (7) hypothalamic areas, particularly dorsal and lateral hypothalamic areas; (8) preoptic areas; (9) anarea dorso-lateral to the inferior olivary complex and medial to the lateral reticular nucleus; and (10) raphe nuclei; particularly nucleus linearis intermedius, centralis superior, pontis and magnus.
Projections from the entopeduncular nucleus (pallidum) and the lateral hypothalamic area to the lateral habenular nucleus, and from the latter to the dorsal raphe nucleus were also found.
HRP deposits confined to the lateral half of the lateral habenular nucleus labeled a multitude of cells in the entopeduncular nucleus. Numerous labeled cells also appeared in such cases in the lateral hypothalamus, indicating that the lateral habenular nucleus is a major convergence point of projections from these otherwise apparently quite separate cell regions. HRP injected into the medial part of the lateral habenular nucleus labeled cells in the same regions, but more in the diagonal band and fewer in the entopeduncular nucleus than were labeled by more lateral injections. The contrast suggests that the projections from the basal forebrain and entopeduncular nucleus to the lateral habenular nucleus are somewhat topographically organized.
The neurons of the lateral habenular nucleus (LH) were divided into four groups.
These cells also send fibers through the stria medullaris to the lateral habenular nucleus and mediodorsal thalamic nucleus.
Cells labeled by injections of the MPS transported isotope to thalamic nuclei (ventral anterior, VApc, ventral lateral, VLo and VLm, and the centromedian, CM), the pedunculopontine nucleus (PPN), and the lateral habenular nucleus (Hbl).
After HRP injections in the medianus raphe nucleus labelled neurons appeared in the lateral habenular nucleus and parafascicular nucleus. Labelled neurons were also found in the lateral habenular nucleus after injections in either the dorsalis raphe nucleus or the caudal central gray substance. The present results suggest also that in the rat the lateral habenular nucleus might be the link between basal forbrain inputs and the limbic midbrain area.
In the diencephalon terminal fields lay in the dorsal hypothalamus, the subthalamus, lateral habenular nucleus, and the following thalamic nuclei: nucleus reticularis, ventralis anterior, centralis medialis, centralis superior lateralis, centralis inferior, submedius, medialis dorsalis and centrum medianum.
A continuous rhythmic discharge in the subthalamic nucleus (STN), lateral habenular nucleus, and ventral tegmental area (VTA) is associated with tremor in cats lightly anesthetized with barbiturate.
The epithalamus does not show any striking structural differences, except some architectonic differentiation in the lateral habenular nucleus.
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